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KMID : 1146620130020010001
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Journal of Korean Academy of Advanced General Dentistry
2013 Volume.2 No. 1 p.1 ~ p.10
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Bone Regeneration of Mesenchymal Stem Cells Isolated from Umbilical Cord Blood in Calvarial Defect of Athymic Mouse
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Yoon Jung-Hyun
Lee Jin-Yong
Lim Jae-Hyung
Lee Eui-Seok
Seo Byoung-Moo
Jang Hyun-Seok
Kwon Jong-Jin
Rim Jae-Suk
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Abstract
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It has been suggested that umbilical cord blood (UCB) contains multipotent stem cells. Some authors suggest that within UCB are cells with potential for differentiation down mesenchymal lineages and UCB is one of the readily available sources of stem cells that may be used for bone regeneration. Isolation and characterization of these cells has been accomplished in some centers. Differentiation of these cells down multiple lineages has been documented. The use of UCB derived stem cells was investigated in this animal studies. Polycaprolactone?20% tricalcium phosphate (PCL?TCP) polymers allow for cellular adherence and differentiation and are adequate scaffolds for bone regeneration in tissue engineering strategies. The purpose of this study was to evaluate the potential of MSCs from UCB seeded onto PCL?TCP composite scaffolds for bone tissue engineering. MSCs were isolated from UCB. MSCs from UCB seeded onto PCL?TCP scaffolds were then implanted in calvarial bone defect of the athymic mouse. As a control, in each mouse was treated with scaffold only. At six and twelve weeks post?implantation, histological examinations were performed. MSCs from UCB with PCL?TCP scaffolds did not exhibited bone regeneration in calvarial bone defects in athymic mouse. Histological examination did not reveal the presence of regenerated bone of the defect. MSCs from UCB should be considered further applications in tissue?engineered bone regeneration.
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KEYWORD
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Umbilical cord blood derived mesenchymal stem cells, Mesenchymal stem cells, Umbilical cord blood, PCL?TCP scaffold, Bone regeneration, Athymic mouse
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